The fraction containing the purified PLA2 was lyophilized. 2.5. BoaPLI. Principal framework of BoaPLI recommended around molecular mass of 22 kDa. When BoaPLI was incubated with Asp-49 and Lys-49 PLA2 there is no severe transformation in its dichroism range, recommending a non-covalent connections. The enzymatic assay demonstrated a dose-dependent inhibition, up to 48.2%, when BoaPLI … Continue reading The fraction containing the purified PLA2 was lyophilized